primary human dermal microvascular ec Search Results


hdmecs  (PromoCell)
96
PromoCell hdmecs
Hdmecs, supplied by PromoCell, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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5×10 5 primary human dermal microvascular ecs  (Lonza)
90
Lonza 5×10 5 primary human dermal microvascular ecs
5×10 5 Primary Human Dermal Microvascular Ecs, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human dermal microvascular endothelial cells  (BioWhittaker Molecular Applications)
90
BioWhittaker Molecular Applications human dermal microvascular endothelial cells
Human Dermal Microvascular Endothelial Cells, supplied by BioWhittaker Molecular Applications, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human microvascular dermal ec  (Upstate Biotechnology Inc)
90
Upstate Biotechnology Inc human microvascular dermal ec
Human Microvascular Dermal Ec, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primary human dermal microvascular endothelial cells hdbec  (Lonza)
90
Lonza primary human dermal microvascular endothelial cells hdbec
Primary Human Dermal Microvascular Endothelial Cells Hdbec, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hmvec cc-2516  (Cambrex)
90
Cambrex hmvec cc-2516
Hmvec Cc 2516, supplied by Cambrex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dermal-derived human microvascular ec (hmec)  (Lonza)
90
Lonza dermal-derived human microvascular ec (hmec)
Dermal Derived Human Microvascular Ec (Hmec), supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fibronectin primary human dermal microvascular endothelial cells (hdmec)  (Cambrex)
90
Cambrex fibronectin primary human dermal microvascular endothelial cells (hdmec)
Fibronectin Primary Human Dermal Microvascular Endothelial Cells (Hdmec), supplied by Cambrex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human dermal microvascular ec hemveca-d  (Lonza)
90
Lonza human dermal microvascular ec hemveca-d
(A) Fluorescence microscope representative pictures of 2D co-culture in vitro model composed with different breast tumour cells and endothelial cells (ECs) in a 1:1 ratio. (B) Quantification of the ratio of the cells in co-culture after 48 hours by flow cytometry (1,000,000 cells of each cell type were initially seeded in each well). (C) Percentage of MDA-MB-231, MDA-MB-436 and MCF-10A cells in G0/G1, S and G2/M phase 48 hours after single culture or in co-culture with human dermal <t>microvascular</t> EC (HMEVCa-D) cells. (D) Sorting plots and gates were used for Ki67 analysis. The plot in red showed the percentage of Ki67 positive cells when MDA-MB-231 cells were cultured alone. The plot blue showed the percentage of Ki67 positive cells when MDA-MB-231 cells were co-cultured with HMEVCa-D for 48 hours.
Human Dermal Microvascular Ec Hemveca D, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primary neonatal foreskin human dermal microvascular endothelial cells (neonatal phdmecs)  (Lonza)
90
Lonza primary neonatal foreskin human dermal microvascular endothelial cells (neonatal phdmecs)
(A) Fluorescence microscope representative pictures of 2D co-culture in vitro model composed with different breast tumour cells and endothelial cells (ECs) in a 1:1 ratio. (B) Quantification of the ratio of the cells in co-culture after 48 hours by flow cytometry (1,000,000 cells of each cell type were initially seeded in each well). (C) Percentage of MDA-MB-231, MDA-MB-436 and MCF-10A cells in G0/G1, S and G2/M phase 48 hours after single culture or in co-culture with human dermal <t>microvascular</t> EC (HMEVCa-D) cells. (D) Sorting plots and gates were used for Ki67 analysis. The plot in red showed the percentage of Ki67 positive cells when MDA-MB-231 cells were cultured alone. The plot blue showed the percentage of Ki67 positive cells when MDA-MB-231 cells were co-cultured with HMEVCa-D for 48 hours.
Primary Neonatal Foreskin Human Dermal Microvascular Endothelial Cells (Neonatal Phdmecs), supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human microvascular dermal ec  (CellSystems Biotechnologie Vertrieb GmbH)
90
CellSystems Biotechnologie Vertrieb GmbH human microvascular dermal ec
(A) Fluorescence microscope representative pictures of 2D co-culture in vitro model composed with different breast tumour cells and endothelial cells (ECs) in a 1:1 ratio. (B) Quantification of the ratio of the cells in co-culture after 48 hours by flow cytometry (1,000,000 cells of each cell type were initially seeded in each well). (C) Percentage of MDA-MB-231, MDA-MB-436 and MCF-10A cells in G0/G1, S and G2/M phase 48 hours after single culture or in co-culture with human dermal <t>microvascular</t> EC (HMEVCa-D) cells. (D) Sorting plots and gates were used for Ki67 analysis. The plot in red showed the percentage of Ki67 positive cells when MDA-MB-231 cells were cultured alone. The plot blue showed the percentage of Ki67 positive cells when MDA-MB-231 cells were co-cultured with HMEVCa-D for 48 hours.
Human Microvascular Dermal Ec, supplied by CellSystems Biotechnologie Vertrieb GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primary dneo-neonatal human dermal microvascular endothelial cells (mvecs)  (Lonza)
90
Lonza primary dneo-neonatal human dermal microvascular endothelial cells (mvecs)
(A) Fluorescence microscope representative pictures of 2D co-culture in vitro model composed with different breast tumour cells and endothelial cells (ECs) in a 1:1 ratio. (B) Quantification of the ratio of the cells in co-culture after 48 hours by flow cytometry (1,000,000 cells of each cell type were initially seeded in each well). (C) Percentage of MDA-MB-231, MDA-MB-436 and MCF-10A cells in G0/G1, S and G2/M phase 48 hours after single culture or in co-culture with human dermal <t>microvascular</t> EC (HMEVCa-D) cells. (D) Sorting plots and gates were used for Ki67 analysis. The plot in red showed the percentage of Ki67 positive cells when MDA-MB-231 cells were cultured alone. The plot blue showed the percentage of Ki67 positive cells when MDA-MB-231 cells were co-cultured with HMEVCa-D for 48 hours.
Primary Dneo Neonatal Human Dermal Microvascular Endothelial Cells (Mvecs), supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) Fluorescence microscope representative pictures of 2D co-culture in vitro model composed with different breast tumour cells and endothelial cells (ECs) in a 1:1 ratio. (B) Quantification of the ratio of the cells in co-culture after 48 hours by flow cytometry (1,000,000 cells of each cell type were initially seeded in each well). (C) Percentage of MDA-MB-231, MDA-MB-436 and MCF-10A cells in G0/G1, S and G2/M phase 48 hours after single culture or in co-culture with human dermal microvascular EC (HMEVCa-D) cells. (D) Sorting plots and gates were used for Ki67 analysis. The plot in red showed the percentage of Ki67 positive cells when MDA-MB-231 cells were cultured alone. The plot blue showed the percentage of Ki67 positive cells when MDA-MB-231 cells were co-cultured with HMEVCa-D for 48 hours.

Journal: Oncotarget

Article Title: Association of breast carcinoma growth with a non-canonical axis of IFNγ/IDO1/TSP1

doi: 10.18632/oncotarget.18781

Figure Lengend Snippet: (A) Fluorescence microscope representative pictures of 2D co-culture in vitro model composed with different breast tumour cells and endothelial cells (ECs) in a 1:1 ratio. (B) Quantification of the ratio of the cells in co-culture after 48 hours by flow cytometry (1,000,000 cells of each cell type were initially seeded in each well). (C) Percentage of MDA-MB-231, MDA-MB-436 and MCF-10A cells in G0/G1, S and G2/M phase 48 hours after single culture or in co-culture with human dermal microvascular EC (HMEVCa-D) cells. (D) Sorting plots and gates were used for Ki67 analysis. The plot in red showed the percentage of Ki67 positive cells when MDA-MB-231 cells were cultured alone. The plot blue showed the percentage of Ki67 positive cells when MDA-MB-231 cells were co-cultured with HMEVCa-D for 48 hours.

Article Snippet: A panel of human ECs includes human dermal microvascular EC (HEMVEca-D) (Lonza Biologies plc.

Techniques: Fluorescence, Microscopy, Co-Culture Assay, In Vitro, Flow Cytometry, Cell Culture

(A) ELISA assay shows that IFNγ induces a reduction in HMEVCa-D cells secreting TSP1 into extracellular space with IC 50 =10ng/ml. (B) HMEVCa-D cells were treated with the medium implemented with different concentrations of tryptophan. Addition of tryptophan for 72 hours resulted in an increase in TSP1 expression in ECs with IE 50 of approximately 5μM. (C) ELISA assay reveals that the MDA-MB-231 cell-conditioned medium (normal or low glucose) induced a significantly increase in intracellular L-kynurenine protein levels in HMEVCa-D cells, an indication of tryptophan degradation, which is reversed by IDO1 siRNA and IDO1 inhibitor-1-Methyl-tryptophan (1MT). (D) A possible non-canonical role of IFNγ/IDO1/TSP1 axis in microvascular niche-dominated tumour dormancy of breast invasive ductal carcinoma cells. High levels of TSP1 in stroma suppressed the IDC cells directly. The antitumor effects result in recognition and elimination of the stromal TSP1 by the IDC cells, triggering an increase in IFNγ-stimulated IDO1 levels in the adjacent vascular ECs. The vascular IDO1 serves as the major negative regulator of the stromal TSP1 proteins by degrading tryptophan, one essential amino acid for TSP1 synthesis, which ultimately leads to a reduction in the stromal TSP1 proteins and the escape of the IDC cells from siege of stromal TSP1.

Journal: Oncotarget

Article Title: Association of breast carcinoma growth with a non-canonical axis of IFNγ/IDO1/TSP1

doi: 10.18632/oncotarget.18781

Figure Lengend Snippet: (A) ELISA assay shows that IFNγ induces a reduction in HMEVCa-D cells secreting TSP1 into extracellular space with IC 50 =10ng/ml. (B) HMEVCa-D cells were treated with the medium implemented with different concentrations of tryptophan. Addition of tryptophan for 72 hours resulted in an increase in TSP1 expression in ECs with IE 50 of approximately 5μM. (C) ELISA assay reveals that the MDA-MB-231 cell-conditioned medium (normal or low glucose) induced a significantly increase in intracellular L-kynurenine protein levels in HMEVCa-D cells, an indication of tryptophan degradation, which is reversed by IDO1 siRNA and IDO1 inhibitor-1-Methyl-tryptophan (1MT). (D) A possible non-canonical role of IFNγ/IDO1/TSP1 axis in microvascular niche-dominated tumour dormancy of breast invasive ductal carcinoma cells. High levels of TSP1 in stroma suppressed the IDC cells directly. The antitumor effects result in recognition and elimination of the stromal TSP1 by the IDC cells, triggering an increase in IFNγ-stimulated IDO1 levels in the adjacent vascular ECs. The vascular IDO1 serves as the major negative regulator of the stromal TSP1 proteins by degrading tryptophan, one essential amino acid for TSP1 synthesis, which ultimately leads to a reduction in the stromal TSP1 proteins and the escape of the IDC cells from siege of stromal TSP1.

Article Snippet: A panel of human ECs includes human dermal microvascular EC (HEMVEca-D) (Lonza Biologies plc.

Techniques: Enzyme-linked Immunosorbent Assay, Expressing